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D4513
Sigma
Deoxyribonuclease I from bovine pancreas
Type II-S, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein
| Synonym: | Deoxyribonucleate 5′-oligonucleotido-hydrolase, DNase I |
| CAS Number: | 9003-98-9 |
| Enzyme Commission (EC) Number: | 3.1.21.1 ( BRENDA | IUBMB ) |
| EC Number: | 232-667-0 |
| MDL number: | MFCD00130918 |
Description
| Analysis Note | Protein determined by biuret. |
| Application | Used for the removal of DNA from protein samples. |
| Preparation Note | Derived from New Zealand-sourced pancreas |
| Unit Definition | One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. [Mg2+] = 4.2 mM. |
| Physical form | Lyophilized powder containing calcium chloride |
Properties
| sterility | sterile-filtered |
| type | Type II-S |
| form | lyophilized powder |
| mol wt | mol wt ~31 kDa |
| purified by | chromatography |
| composition | Protein, ≥80% |
| packaging | vial of ~11 mg protein |
| total impurities | Endotoxin, tested |
| EQP level | Premium |
| foreign activity | Chymotrypsin ≤0.01% |
| Protease ≤0.005% | |
| RNase ≤0.01% | |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
| RTECS | RF0750000 |
Related Products
References
| reference | Cull, M., and McHenry, C.S., Preparation of extracts from prokaryotes. Meth. Enzymol. 182, 147-153, (1990) |
| Molloy, M.P., et al., Proteomic analysis of the Escherichia coli outer membrane. Eur. J. Biochem. 267, 2871-2881, (2000) | |
| Moore, S. The Enzymes 3rd ed.,, (1981) 14, 281 | |
| Laskowski, M. The Enzymes 3rd ed.,, (1971) 4, 289 | |
| Lindberg, U. Biochemistry 6, 335, (1967) | |
| Merck | Merck 13,2923 |
